Construct: shRNA TRCN0000054039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002841.1-458s1c1
DNA Barcode:
GCTGGACTTTGAGACGTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL4 (4635)
Gene Description:
myosin light chain 4
Transcript:
RefSeq NM_001002841.1 (CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGACTTTGAGACGTTCTT
Hairpin Sequence:
5'-CCGG-GCTGGACTTTGAGACGTTCTT-CTCGAG-AAGAACGTCTCAAAGTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGACTTTGAGACGTTCTTCTCGAGAAGAACGTCTCAAAGTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGACTTTGAGACGTTCTTCTCGAGAAGAACGTCTCAAAGTCCAGC-3'

Other clones with same target sequence:

(none)