Construct: shRNA TRCN0000054041

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002841.1-666s1c1
DNA Barcode:
GATGCCAATGGCTGCATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL4 (4635)
Gene Description:
myosin light chain 4
Transcript:
RefSeq NM_001002841.1 (CURRENT)
Match location:
Position 666 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054041)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCCAATGGCTGCATCAAT
Hairpin Sequence:
5'-CCGG-GATGCCAATGGCTGCATCAAT-CTCGAG-ATTGATGCAGCCATTGGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCCAATGGCTGCATCAATCTCGAGATTGATGCAGCCATTGGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCCAATGGCTGCATCAATCTCGAGATTGATGCAGCCATTGGCATC-3'

Other clones with same target sequence:

(none)