Construct: shRNA TRCN0000054050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003248.3-3156s1c1
DNA Barcode:
CCTGAGGACTTCCAAGAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THBS4 (7060)
Gene Description:
thrombospondin 4
Transcript:
RefSeq NM_003248.3 (NON-CURRENT)
Match location:
Position 3156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGGACTTCCAAGAGTTT
Hairpin Sequence:
5'-CCGG-CCTGAGGACTTCCAAGAGTTT-CTCGAG-AAACTCTTGGAAGTCCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGGACTTCCAAGAGTTTCTCGAGAAACTCTTGGAAGTCCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGGACTTCCAAGAGTTTCTCGAGAAACTCTTGGAAGTCCTCAGG-3'

Other clones with same target sequence:

(none)