Construct: shRNA TRCN0000054089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001795.2-1695s1c1
DNA Barcode:
CCTCACGGATAATCACGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDH5 (1003)
Gene Description:
cadherin 5
Transcript:
RefSeq NM_001795.2 (NON-CURRENT)
Match location:
Position 1695 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACGGATAATCACGATAA
Hairpin Sequence:
5'-CCGG-CCTCACGGATAATCACGATAA-CTCGAG-TTATCGTGATTATCCGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACGGATAATCACGATAACTCGAGTTATCGTGATTATCCGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACGGATAATCACGATAACTCGAGTTATCGTGATTATCCGTGAGG-3'

Other clones with same target sequence:

(none)