Construct: shRNA TRCN0000054131

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018557.1-11190s1c1
DNA Barcode:
CCAGAAATGTATCCCAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRP1B (53353)
Gene Description:
LDL receptor related protein 1B
Transcript:
RefSeq NM_018557.1 (NON-CURRENT)
Match location:
Position 11190 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054131)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAATGTATCCCAGTAAA
Hairpin Sequence:
5'-CCGG-CCAGAAATGTATCCCAGTAAA-CTCGAG-TTTACTGGGATACATTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAATGTATCCCAGTAAACTCGAGTTTACTGGGATACATTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAATGTATCCCAGTAAACTCGAGTTTACTGGGATACATTTCTGG-3'

Other clones with same target sequence:

(none)