Construct: shRNA TRCN0000054153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022124.2-10638s1c1
DNA Barcode:
GCATCTGACCTCTACCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDH23 (64072)
Gene Description:
cadherin related 23
Transcript:
RefSeq NM_022124.2 (NON-CURRENT)
Match location:
Position 10638 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTGACCTCTACCTTCAT
Hairpin Sequence:
5'-CCGG-GCATCTGACCTCTACCTTCAT-CTCGAG-ATGAAGGTAGAGGTCAGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTGACCTCTACCTTCATCTCGAGATGAAGGTAGAGGTCAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTGACCTCTACCTTCATCTCGAGATGAAGGTAGAGGTCAGATGC-3'

Other clones with same target sequence:

(none)