Construct: shRNA TRCN0000054227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001796.2-565s1c1
DNA Barcode:
GCTGGGACCATATTTCAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDH8 (1006)
Gene Description:
cadherin 8
Transcript:
RefSeq NM_001796.2 (NON-CURRENT)
Match location:
Position 565 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGACCATATTTCAAATA
Hairpin Sequence:
5'-CCGG-GCTGGGACCATATTTCAAATA-CTCGAG-TATTTGAAATATGGTCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGACCATATTTCAAATACTCGAGTATTTGAAATATGGTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGACCATATTTCAAATACTCGAGTATTTGAAATATGGTCCCAGC-3'

Other clones with same target sequence:

(none)