Construct: shRNA TRCN0000054294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007351.2-677s1c1
DNA Barcode:
CGAAACAACTAGAGGAAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MMRN1 (22915)
Gene Description:
multimerin 1
Transcript:
RefSeq NM_007351.2 (NON-CURRENT)
Match location:
Position 677 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAACAACTAGAGGAAAGAA
Hairpin Sequence:
5'-CCGG-CGAAACAACTAGAGGAAAGAA-CTCGAG-TTCTTTCCTCTAGTTGTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAACAACTAGAGGAAAGAACTCGAGTTCTTTCCTCTAGTTGTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAACAACTAGAGGAAAGAACTCGAGTTCTTTCCTCTAGTTGTTTCG-3'

Other clones with same target sequence:

(none)