Construct: shRNA TRCN0000054308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006159.1-940s1c1
DNA Barcode:
CGAGAATTTGAGTCCTGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NELL2 (4753)
Gene Description:
neural EGFL like 2
Transcript:
RefSeq NM_006159.1 (NON-CURRENT)
Match location:
Position 940 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAATTTGAGTCCTGGATA
Hairpin Sequence:
5'-CCGG-CGAGAATTTGAGTCCTGGATA-CTCGAG-TATCCAGGACTCAAATTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAATTTGAGTCCTGGATACTCGAGTATCCAGGACTCAAATTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAATTTGAGTCCTGGATACTCGAGTATCCAGGACTCAAATTCTCG-3'

Other clones with same target sequence:

  1. TRCN0000292014