Construct: shRNA TRCN0000054340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025176.3-778s1c1
DNA Barcode:
GCAAAGTGAGTCTTGAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NINL (22981)
Gene Description:
ninein like
Transcript:
RefSeq NM_025176.3 (NON-CURRENT)
Match location:
Position 778 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGTGAGTCTTGAGGAAT
Hairpin Sequence:
5'-CCGG-GCAAAGTGAGTCTTGAGGAAT-CTCGAG-ATTCCTCAAGACTCACTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGTGAGTCTTGAGGAATCTCGAGATTCCTCAAGACTCACTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGTGAGTCTTGAGGAATCTCGAGATTCCTCAAGACTCACTTTGC-3'

Other clones with same target sequence:

(none)