Construct: shRNA TRCN0000054383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008951.1-475s1c1
DNA Barcode:
GCCTTTGTGAACACACTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmd4 (19185)
Gene Description:
proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Transcript:
RefSeq NM_008951.1 (NON-CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTGTGAACACACTGAAT
Hairpin Sequence:
5'-CCGG-GCCTTTGTGAACACACTGAAT-CTCGAG-ATTCAGTGTGTTCACAAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTGTGAACACACTGAATCTCGAGATTCAGTGTGTTCACAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTGTGAACACACTGAATCTCGAGATTCAGTGTGTTCACAAAGGC-3'

Other clones with same target sequence:

(none)