Construct: shRNA TRCN0000054536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011282.1-6882s1c1
DNA Barcode:
CCCACTTTCCATAACATTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ros1 (19886)
Gene Description:
Ros1 proto-oncogene
Transcript:
RefSeq NM_011282.1 (NON-CURRENT)
Match location:
Position 6882 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTTTCCATAACATTCAA
Hairpin Sequence:
5'-CCGG-CCCACTTTCCATAACATTCAA-CTCGAG-TTGAATGTTATGGAAAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTTTCCATAACATTCAACTCGAGTTGAATGTTATGGAAAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTTTCCATAACATTCAACTCGAGTTGAATGTTATGGAAAGTGGG-3'

Other clones with same target sequence:

(none)