Construct: shRNA TRCN0000054576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011349.2-745s1c1
DNA Barcode:
CCTCATCAATGAGGAACTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema3f (20350)
Gene Description:
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Transcript:
RefSeq NM_011349.2 (NON-CURRENT)
Match location:
Position 745 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATCAATGAGGAACTCTA
Hairpin Sequence:
5'-CCGG-CCTCATCAATGAGGAACTCTA-CTCGAG-TAGAGTTCCTCATTGATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATCAATGAGGAACTCTACTCGAGTAGAGTTCCTCATTGATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATCAATGAGGAACTCTACTCGAGTAGAGTTCCTCATTGATGAGG-3'

Other clones with same target sequence:

(none)