Construct: shRNA TRCN0000054590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009024.1-596s1c1
DNA Barcode:
GAGCAGCAGTTCCGAAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rara (19401)
Gene Description:
retinoic acid receptor, alpha
Transcript:
RefSeq NM_009024.1 (NON-CURRENT)
Match location:
Position 596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGCAGTTCCGAAGAGAT
Hairpin Sequence:
5'-CCGG-GAGCAGCAGTTCCGAAGAGAT-CTCGAG-ATCTCTTCGGAACTGCTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGCAGTTCCGAAGAGATCTCGAGATCTCTTCGGAACTGCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGCAGTTCCGAAGAGATCTCGAGATCTCTTCGGAACTGCTGCTC-3'

Other clones with same target sequence:

  1. TRCN0000301241