Construct: shRNA TRCN0000054614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009689.1-247s1c1
DNA Barcode:
CCTACCGAGAACGAGCCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Birc5 (11799)
Gene Description:
baculoviral IAP repeat-containing 5
Transcript:
RefSeq NM_009689.1 (NON-CURRENT)
Match location:
Position 247 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCGAGAACGAGCCTGAT
Hairpin Sequence:
5'-CCGG-CCTACCGAGAACGAGCCTGAT-CTCGAG-ATCAGGCTCGTTCTCGGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCGAGAACGAGCCTGATCTCGAGATCAGGCTCGTTCTCGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCGAGAACGAGCCTGATCTCGAGATCAGGCTCGTTCTCGGTAGG-3'

Other clones with same target sequence:

(none)