Construct: shRNA TRCN0000054669
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_023240.2-880s1c1
- DNA Barcode:
- CGAGGAGGAGATCACCAAATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Eef1d (66656)
- Gene Description:
- eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
- Transcript:
- RefSeq NM_023240.2 (NON-CURRENT)
- Match location:
- Position 880 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000054669)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGAGGAGGAGATCACCAAATT
- Hairpin Sequence:
-
5'-CCGG-CGAGGAGGAGATCACCAAATT-CTCGAG-AATTTGGTGATCTCCTCCTCG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGAGGAGGAGATCACCAAATTCTCGAGAATTTGGTGATCTCCTCCTCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGAGGAGGAGATCACCAAATTCTCGAGAATTTGGTGATCTCCTCCTCG-3'
Other clones with same target sequence:
-
TRCN0000301262
