Construct: shRNA TRCN0000054712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019680.1-784s1c1
DNA Barcode:
GCATGCCTATACGGAAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elf4 (56501)
Gene Description:
E74-like factor 4 (ets domain transcription factor)
Transcript:
RefSeq NM_019680.1 (NON-CURRENT)
Match location:
Position 784 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGCCTATACGGAAGAAAT
Hairpin Sequence:
5'-CCGG-GCATGCCTATACGGAAGAAAT-CTCGAG-ATTTCTTCCGTATAGGCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGCCTATACGGAAGAAATCTCGAGATTTCTTCCGTATAGGCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGCCTATACGGAAGAAATCTCGAGATTTCTTCCGTATAGGCATGC-3'

Other clones with same target sequence:

(none)