Construct: shRNA TRCN0000054723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011785.2-1326s1c1
DNA Barcode:
CATCTGAAACAGACACCCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akt3 (23797)
Gene Description:
thymoma viral proto-oncogene 3
Transcript:
RefSeq NM_011785.2 (NON-CURRENT)
Match location:
Position 1326 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTGAAACAGACACCCGAT
Hairpin Sequence:
5'-CCGG-CATCTGAAACAGACACCCGAT-CTCGAG-ATCGGGTGTCTGTTTCAGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTGAAACAGACACCCGATCTCGAGATCGGGTGTCTGTTTCAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTGAAACAGACACCCGATCTCGAGATCGGGTGTCTGTTTCAGATG-3'

Other clones with same target sequence:

(none)