Construct: shRNA TRCN0000054739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019776.1-433s1c1
DNA Barcode:
CGCAAGAAGCTGATTGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snd1 (56463)
Gene Description:
staphylococcal nuclease and tudor domain containing 1
Transcript:
RefSeq NM_019776.1 (NON-CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAGAAGCTGATTGGGAAA
Hairpin Sequence:
5'-CCGG-CGCAAGAAGCTGATTGGGAAA-CTCGAG-TTTCCCAATCAGCTTCTTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAGAAGCTGATTGGGAAACTCGAGTTTCCCAATCAGCTTCTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAGAAGCTGATTGGGAAACTCGAGTTTCCCAATCAGCTTCTTGCG-3'

Other clones with same target sequence:

(none)