Construct: shRNA TRCN0000054793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008625.1-251s1c1
DNA Barcode:
CGGAAGCTGAATCCCAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrc1 (17533)
Gene Description:
mannose receptor, C type 1
Transcript:
RefSeq NM_008625.1 (NON-CURRENT)
Match location:
Position 251 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGCTGAATCCCAGAAAT
Hairpin Sequence:
5'-CCGG-CGGAAGCTGAATCCCAGAAAT-CTCGAG-ATTTCTGGGATTCAGCTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGCTGAATCCCAGAAATCTCGAGATTTCTGGGATTCAGCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGCTGAATCCCAGAAATCTCGAGATTTCTGGGATTCAGCTTCCG-3'

Other clones with same target sequence:

(none)