Construct: shRNA TRCN0000054971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019713.2-541s1c1
DNA Barcode:
GCTATTAAGCATCTGCATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf1 (56289)
Gene Description:
Ras association (RalGDS/AF-6) domain family member 1
Transcript:
RefSeq NM_019713.2 (NON-CURRENT)
Match location:
Position 541 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTAAGCATCTGCATGTT
Hairpin Sequence:
5'-CCGG-GCTATTAAGCATCTGCATGTT-CTCGAG-AACATGCAGATGCTTAATAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTAAGCATCTGCATGTTCTCGAGAACATGCAGATGCTTAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTAAGCATCTGCATGTTCTCGAGAACATGCAGATGCTTAATAGC-3'

Other clones with same target sequence:

(none)