Construct: shRNA TRCN0000054973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149500.5-3216s1c1
DNA Barcode:
CCAGTATCCTTGGTTGTGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rap1gap (110351)
Gene Description:
Rap1 GTPase-activating protein
Transcript:
RefSeq XM_149500.5 (NON-CURRENT)
Match location:
Position 3216 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTATCCTTGGTTGTGGAT
Hairpin Sequence:
5'-CCGG-CCAGTATCCTTGGTTGTGGAT-CTCGAG-ATCCACAACCAAGGATACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTATCCTTGGTTGTGGATCTCGAGATCCACAACCAAGGATACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTATCCTTGGTTGTGGATCTCGAGATCCACAACCAAGGATACTGG-3'

Other clones with same target sequence:

  1. TRCN0000309892