Construct: shRNA TRCN0000054976

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149500.5-523s1c1
DNA Barcode:
CCCTACCCTAAGAACACAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rap1gap (110351)
Gene Description:
Rap1 GTPase-activating protein
Transcript:
RefSeq XM_149500.5 (NON-CURRENT)
Match location:
Position 523 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054976)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACCCTAAGAACACAGAT
Hairpin Sequence:
5'-CCGG-CCCTACCCTAAGAACACAGAT-CTCGAG-ATCTGTGTTCTTAGGGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACCCTAAGAACACAGATCTCGAGATCTGTGTTCTTAGGGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACCCTAAGAACACAGATCTCGAGATCTGTGTTCTTAGGGTAGGG-3'

Other clones with same target sequence:

(none)