Construct: shRNA TRCN0000055028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183308.1-1085s1c1
DNA Barcode:
GCGACCATATATGATAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pon2 (330260)
Gene Description:
paraoxonase 2
Transcript:
RefSeq NM_183308.1 (NON-CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACCATATATGATAGGAAA
Hairpin Sequence:
5'-CCGG-GCGACCATATATGATAGGAAA-CTCGAG-TTTCCTATCATATATGGTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACCATATATGATAGGAAACTCGAGTTTCCTATCATATATGGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACCATATATGATAGGAAACTCGAGTTTCCTATCATATATGGTCGC-3'

Other clones with same target sequence:

(none)