Construct: shRNA TRCN0000055060

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008630.1-127s1c1
DNA Barcode:
GCAAACAATGCAAATGTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mt2 (17750)
Gene Description:
metallothionein 2
Transcript:
RefSeq NM_008630.1 (NON-CURRENT)
Match location:
Position 127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055060)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACAATGCAAATGTACTT
Hairpin Sequence:
5'-CCGG-GCAAACAATGCAAATGTACTT-CTCGAG-AAGTACATTTGCATTGTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACAATGCAAATGTACTTCTCGAGAAGTACATTTGCATTGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACAATGCAAATGTACTTCTCGAGAAGTACATTTGCATTGTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000331990