Construct: shRNA TRCN0000055064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023138.3-1022s1c1
DNA Barcode:
CCACCTGATGCCAAGGAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map2k2 (26396)
Gene Description:
mitogen-activated protein kinase kinase 2
Transcript:
RefSeq NM_023138.3 (NON-CURRENT)
Match location:
Position 1022 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTGATGCCAAGGAACTA
Hairpin Sequence:
5'-CCGG-CCACCTGATGCCAAGGAACTA-CTCGAG-TAGTTCCTTGGCATCAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTGATGCCAAGGAACTACTCGAGTAGTTCCTTGGCATCAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTGATGCCAAGGAACTACTCGAGTAGTTCCTTGGCATCAGGTGG-3'

Other clones with same target sequence:

  1. TRCN0000288077