Construct: shRNA TRCN0000055177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178907.1-576s1c1
DNA Barcode:
CGGCAAGGTACTGGAGTGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapkapk3 (102626)
Gene Description:
mitogen-activated protein kinase-activated protein kinase 3
Transcript:
RefSeq NM_178907.1 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAGGTACTGGAGTGCTA
Hairpin Sequence:
5'-CCGG-CGGCAAGGTACTGGAGTGCTA-CTCGAG-TAGCACTCCAGTACCTTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAGGTACTGGAGTGCTACTCGAGTAGCACTCCAGTACCTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAGGTACTGGAGTGCTACTCGAGTAGCACTCCAGTACCTTGCCG-3'

Other clones with same target sequence:

(none)