Construct: shRNA TRCN0000055224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011951.1-1049s1c1
DNA Barcode:
CTCAGAGTCTGCAAGAAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk14 (26416)
Gene Description:
mitogen-activated protein kinase 14
Transcript:
RefSeq NM_011951.1 (NON-CURRENT)
Match location:
Position 1049 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGAGTCTGCAAGAAACTA
Hairpin Sequence:
5'-CCGG-CTCAGAGTCTGCAAGAAACTA-CTCGAG-TAGTTTCTTGCAGACTCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGAGTCTGCAAGAAACTACTCGAGTAGTTTCTTGCAGACTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGAGTCTGCAAGAAACTACTCGAGTAGTTTCTTGCAGACTCTGAG-3'

Other clones with same target sequence:

(none)