Construct: shRNA TRCN0000055269

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145541.3-382s1c1
DNA Barcode:
GCTCAGTCTACGTTTAATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rap1a (109905)
Gene Description:
RAS-related protein 1a
Transcript:
RefSeq NM_145541.3 (NON-CURRENT)
Match location:
Position 382 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055269)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGTCTACGTTTAATGAT
Hairpin Sequence:
5'-CCGG-GCTCAGTCTACGTTTAATGAT-CTCGAG-ATCATTAAACGTAGACTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGTCTACGTTTAATGATCTCGAGATCATTAAACGTAGACTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGTCTACGTTTAATGATCTCGAGATCATTAAACGTAGACTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000317277