Construct: shRNA TRCN0000055273

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016961.2-2358s1c1
DNA Barcode:
CCACAGAACTTCACCTTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk9 (26420)
Gene Description:
mitogen-activated protein kinase 9
Transcript:
RefSeq NM_016961.2 (NON-CURRENT)
Match location:
Position 2358 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055273)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGAACTTCACCTTCTTT
Hairpin Sequence:
5'-CCGG-CCACAGAACTTCACCTTCTTT-CTCGAG-AAAGAAGGTGAAGTTCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGAACTTCACCTTCTTTCTCGAGAAAGAAGGTGAAGTTCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGAACTTCACCTTCTTTCTCGAGAAAGAAGGTGAAGTTCTGTGG-3'

Other clones with same target sequence:

(none)