Construct: shRNA TRCN0000055367

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023805.2-606s1c1
DNA Barcode:
CCTGTTGTCTAGCTATTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc38a3 (76257)
Gene Description:
solute carrier family 38, member 3
Transcript:
RefSeq NM_023805.2 (NON-CURRENT)
Match location:
Position 606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055367)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTGTCTAGCTATTCCAT
Hairpin Sequence:
5'-CCGG-CCTGTTGTCTAGCTATTCCAT-CTCGAG-ATGGAATAGCTAGACAACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTGTCTAGCTATTCCATCTCGAGATGGAATAGCTAGACAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTGTCTAGCTATTCCATCTCGAGATGGAATAGCTAGACAACAGG-3'

Other clones with same target sequence:

(none)