Construct: shRNA TRCN0000055429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003328.x-646s1c2
DNA Barcode:
CCGTATTTATGGGAGCTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TXK (7294)
Gene Description:
TXK tyrosine kinase
Transcript:
RefSeq NM_003328.1 (NON-CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTATTTATGGGAGCTAGAA
Hairpin Sequence:
5'-CCGG-CCGTATTTATGGGAGCTAGAA-CTCGAG-TTCTAGCTCCCATAAATACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTATTTATGGGAGCTAGAACTCGAGTTCTAGCTCCCATAAATACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTATTTATGGGAGCTAGAACTCGAGTTCTAGCTCCCATAAATACGG-3'

Other clones with same target sequence:

  1. TRCN0000009998