Construct: shRNA TRCN0000055433
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000294.x-673s1c2
- DNA Barcode:
- CTAGCGCCAGAGATCCTTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PHKG2 (5261)
- Gene Description:
- phosphorylase kinase catalytic subunit gamma 2
- Transcript:
- RefSeq NM_000294.1 (NON-CURRENT)
- Match location:
- Position 673 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000055433)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTAGCGCCAGAGATCCTTAAA
- Hairpin Sequence:
-
5'-CCGG-CTAGCGCCAGAGATCCTTAAA-CTCGAG-TTTAAGGATCTCTGGCGCTAG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTAGCGCCAGAGATCCTTAAACTCGAGTTTAAGGATCTCTGGCGCTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTAGCGCCAGAGATCCTTAAACTCGAGTTTAAGGATCTCTGGCGCTAG-3'
Other clones with same target sequence:
-
TRCN0000010068
