Construct: shRNA TRCN0000055522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_031401.7-733s1c1
DNA Barcode:
CCTGTGAAGATGTGGACGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEGF6 (1953)
Gene Description:
multiple EGF like domains 6
Transcript:
RefSeq XM_031401.7 (NON-CURRENT)
Match location:
Position 733 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGAAGATGTGGACGAAT
Hairpin Sequence:
5'-CCGG-CCTGTGAAGATGTGGACGAAT-CTCGAG-ATTCGTCCACATCTTCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGAAGATGTGGACGAATCTCGAGATTCGTCCACATCTTCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGAAGATGTGGACGAATCTCGAGATTCGTCCACATCTTCACAGG-3'

Other clones with same target sequence:

(none)