Construct: shRNA TRCN0000055523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005013.1-674s1c1
DNA Barcode:
CCACAGATTTAGATATGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUCB2 (4925)
Gene Description:
nucleobindin 2
Transcript:
RefSeq NM_005013.1 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGATTTAGATATGCTAA
Hairpin Sequence:
5'-CCGG-CCACAGATTTAGATATGCTAA-CTCGAG-TTAGCATATCTAAATCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGATTTAGATATGCTAACTCGAGTTAGCATATCTAAATCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGATTTAGATATGCTAACTCGAGTTAGCATATCTAAATCTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000300175