Construct: shRNA TRCN0000055584
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_376905.1-2021s1c1
- DNA Barcode:
- GCATTCCCAATGCAGATGTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MEGF9 (1955)
- Gene Description:
- multiple EGF like domains 9
- Transcript:
- RefSeq XM_376905.1 (NON-CURRENT)
- Match location:
- Position 2021 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000055584)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCATTCCCAATGCAGATGTTT
- Hairpin Sequence:
-
5'-CCGG-GCATTCCCAATGCAGATGTTT-CTCGAG-AAACATCTGCATTGGGAATGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCATTCCCAATGCAGATGTTTCTCGAGAAACATCTGCATTGGGAATGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCATTCCCAATGCAGATGTTTCTCGAGAAACATCTGCATTGGGAATGC-3'
Other clones with same target sequence:
-
TRCN0000291337