Construct: shRNA TRCN0000055626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152573.2-2144s1c1
DNA Barcode:
GCTTTCTTAACATACGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASEF (158158)
Gene Description:
RAS and EF-hand domain containing
Transcript:
RefSeq NM_152573.2 (NON-CURRENT)
Match location:
Position 2144 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCTTAACATACGAGAAT
Hairpin Sequence:
5'-CCGG-GCTTTCTTAACATACGAGAAT-CTCGAG-ATTCTCGTATGTTAAGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCTTAACATACGAGAATCTCGAGATTCTCGTATGTTAAGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCTTAACATACGAGAATCTCGAGATTCTCGTATGTTAAGAAAGC-3'

Other clones with same target sequence:

(none)