Construct: shRNA TRCN0000055663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015113.2-9812s1c1
DNA Barcode:
CGTGAGCTTATGTACCGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZZEF1 (23140)
Gene Description:
zinc finger ZZ-type and EF-hand domain containing 1
Transcript:
RefSeq NM_015113.2 (NON-CURRENT)
Match location:
Position 9812 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAGCTTATGTACCGTGAA
Hairpin Sequence:
5'-CCGG-CGTGAGCTTATGTACCGTGAA-CTCGAG-TTCACGGTACATAAGCTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAGCTTATGTACCGTGAACTCGAGTTCACGGTACATAAGCTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAGCTTATGTACCGTGAACTCGAGTTCACGGTACATAAGCTCACG-3'

Other clones with same target sequence:

  1. TRCN0000289438