Construct: shRNA TRCN0000055750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018903.2-1092s1c1
DNA Barcode:
CCATTGATAAAGGGATTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHA12 (56137)
Gene Description:
protocadherin alpha 12
Transcript:
RefSeq NM_018903.2 (NON-CURRENT)
Match location:
Position 1092 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGATAAAGGGATTCCTT
Hairpin Sequence:
5'-CCGG-CCATTGATAAAGGGATTCCTT-CTCGAG-AAGGAATCCCTTTATCAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGATAAAGGGATTCCTTCTCGAGAAGGAATCCCTTTATCAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGATAAAGGGATTCCTTCTCGAGAAGGAATCCCTTTATCAATGG-3'

Other clones with same target sequence:

(none)