Construct: shRNA TRCN0000055827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001102.2-2213s1c1
DNA Barcode:
GCATCGTCAACTACAAGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTN1 (87)
Gene Description:
actinin alpha 1
Transcript:
RefSeq NM_001102.2 (NON-CURRENT)
Match location:
Position 2213 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGTCAACTACAAGCCAA
Hairpin Sequence:
5'-CCGG-GCATCGTCAACTACAAGCCAA-CTCGAG-TTGGCTTGTAGTTGACGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGTCAACTACAAGCCAACTCGAGTTGGCTTGTAGTTGACGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGTCAACTACAAGCCAACTCGAGTTGGCTTGTAGTTGACGATGC-3'

Other clones with same target sequence:

(none)