Construct: shRNA TRCN0000055858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016184.2-564s1c1
DNA Barcode:
GCCCAAAGAATTGGAAGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLEC4A (50856)
Gene Description:
C-type lectin domain family 4 member A
Transcript:
RefSeq NM_016184.2 (NON-CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAAAGAATTGGAAGTCAT
Hairpin Sequence:
5'-CCGG-GCCCAAAGAATTGGAAGTCAT-CTCGAG-ATGACTTCCAATTCTTTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAAAGAATTGGAAGTCATCTCGAGATGACTTCCAATTCTTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAAAGAATTGGAAGTCATCTCGAGATGACTTCCAATTCTTTGGGC-3'

Other clones with same target sequence:

(none)