Construct: shRNA TRCN0000055890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152750.2-585s1c1
DNA Barcode:
CCCTCAGTTATTTCCTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDHR3 (222256)
Gene Description:
cadherin related family member 3
Transcript:
RefSeq NM_152750.2 (NON-CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCAGTTATTTCCTGATTT
Hairpin Sequence:
5'-CCGG-CCCTCAGTTATTTCCTGATTT-CTCGAG-AAATCAGGAAATAACTGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCAGTTATTTCCTGATTTCTCGAGAAATCAGGAAATAACTGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCAGTTATTTCCTGATTTCTCGAGAAATCAGGAAATAACTGAGGG-3'

Other clones with same target sequence:

(none)