Construct: shRNA TRCN0000055892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152750.2-1175s1c1
DNA Barcode:
GACCTAAACAAGTTCTGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDHR3 (222256)
Gene Description:
cadherin related family member 3
Transcript:
RefSeq NM_152750.2 (NON-CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000055892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTAAACAAGTTCTGCTTT
Hairpin Sequence:
5'-CCGG-GACCTAAACAAGTTCTGCTTT-CTCGAG-AAAGCAGAACTTGTTTAGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTAAACAAGTTCTGCTTTCTCGAGAAAGCAGAACTTGTTTAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTAAACAAGTTCTGCTTTCTCGAGAAAGCAGAACTTGTTTAGGTC-3'

Other clones with same target sequence:

(none)