Construct: shRNA TRCN0000056064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003919.1-297s1c1
DNA Barcode:
CCTGGCGAGATTAGTAATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGCE (8910)
Gene Description:
sarcoglycan epsilon
Transcript:
RefSeq NM_003919.1 (NON-CURRENT)
Match location:
Position 297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCGAGATTAGTAATGAT
Hairpin Sequence:
5'-CCGG-CCTGGCGAGATTAGTAATGAT-CTCGAG-ATCATTACTAATCTCGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCGAGATTAGTAATGATCTCGAGATCATTACTAATCTCGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCGAGATTAGTAATGATCTCGAGATCATTACTAATCTCGCCAGG-3'

Other clones with same target sequence:

(none)