Construct: shRNA TRCN0000056106

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016112.2-873s1c1
DNA Barcode:
CCACTACTGGACAGTTTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKD2L1 (9033)
Gene Description:
polycystin 2 like 1, transient receptor potential cation channel
Transcript:
RefSeq NM_016112.2 (NON-CURRENT)
Match location:
Position 873 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056106)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTACTGGACAGTTTGTAT
Hairpin Sequence:
5'-CCGG-CCACTACTGGACAGTTTGTAT-CTCGAG-ATACAAACTGTCCAGTAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTACTGGACAGTTTGTATCTCGAGATACAAACTGTCCAGTAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTACTGGACAGTTTGTATCTCGAGATACAAACTGTCCAGTAGTGG-3'

Other clones with same target sequence:

(none)