Construct: shRNA TRCN0000056108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024948.1-840s1c1
DNA Barcode:
CCCTTGATAGATCCTGTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MINDY3 (80013)
Gene Description:
MINDY lysine 48 deubiquitinase 3
Transcript:
RefSeq NM_024948.1 (NON-CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTGATAGATCCTGTATAT
Hairpin Sequence:
5'-CCGG-CCCTTGATAGATCCTGTATAT-CTCGAG-ATATACAGGATCTATCAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTGATAGATCCTGTATATCTCGAGATATACAGGATCTATCAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTGATAGATCCTGTATATCTCGAGATATACAGGATCTATCAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000289714