Construct: shRNA TRCN0000056118

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001891.1-201s1c1
DNA Barcode:
CCTCTGATCTATCCATTCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSN2 (1447)
Gene Description:
casein beta
Transcript:
RefSeq NM_001891.1 (NON-CURRENT)
Match location:
Position 201 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056118)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGATCTATCCATTCGTT
Hairpin Sequence:
5'-CCGG-CCTCTGATCTATCCATTCGTT-CTCGAG-AACGAATGGATAGATCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGATCTATCCATTCGTTCTCGAGAACGAATGGATAGATCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGATCTATCCATTCGTTCTCGAGAACGAATGGATAGATCAGAGG-3'

Other clones with same target sequence:

(none)