Construct: shRNA TRCN0000056133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018928.2-1640s1c1
DNA Barcode:
GCACTGTAACAGTTCGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGC4 (56098)
Gene Description:
protocadherin gamma subfamily C, 4
Transcript:
RefSeq NM_018928.2 (CURRENT)
Match location:
Position 1640 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGTAACAGTTCGTCTAT
Hairpin Sequence:
5'-CCGG-GCACTGTAACAGTTCGTCTAT-CTCGAG-ATAGACGAACTGTTACAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGTAACAGTTCGTCTATCTCGAGATAGACGAACTGTTACAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGTAACAGTTCGTCTATCTCGAGATAGACGAACTGTTACAGTGC-3'

Other clones with same target sequence:

(none)