Construct: shRNA TRCN0000056145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002857.1-245s1c1
DNA Barcode:
CGGGATGCTTTGAACATTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANXA2 (302)
Gene Description:
annexin A2
Transcript:
RefSeq NM_001002857.1 (NON-CURRENT)
Match location:
Position 245 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATGCTTTGAACATTGAA
Hairpin Sequence:
5'-CCGG-CGGGATGCTTTGAACATTGAA-CTCGAG-TTCAATGTTCAAAGCATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATGCTTTGAACATTGAACTCGAGTTCAATGTTCAAAGCATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATGCTTTGAACATTGAACTCGAGTTCAATGTTCAAAGCATCCCG-3'

Other clones with same target sequence:

  1. TRCN0000289781