Construct: shRNA TRCN0000056163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003735.2-1130s1c1
DNA Barcode:
GCAATGGATAATGCAGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGA12 (26025)
Gene Description:
protocadherin gamma subfamily A, 12
Transcript:
RefSeq NM_003735.2 (NON-CURRENT)
Match location:
Position 1130 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000056163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGGATAATGCAGGATAT
Hairpin Sequence:
5'-CCGG-GCAATGGATAATGCAGGATAT-CTCGAG-ATATCCTGCATTATCCATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGGATAATGCAGGATATCTCGAGATATCCTGCATTATCCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGGATAATGCAGGATATCTCGAGATATCCTGCATTATCCATTGC-3'

Other clones with same target sequence:

(none)